Cytoplasmic lysis buffer (1% TritonX lysis buffer) is somehow lysing my nucleus and releasing my nuclear fraction into the cytoplasmic fraction?

As mentioned above, I am having trouble with my subcellular fractionation of 4T1 cells. For my cytoplasmic lysis buffer, I am using 1% TritonX-100 Lysis buffer (20mM HEPES, 50mM NaCl, 1.5mM...

06 January 2020 9,805 2 View