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Currently I am using HIF1a ab from CST (D5F3M) to probe for HIF1a levels. However, in my hypoxic samples I did not see any induction of HIF1a. I then attempted to rescue the HIF1a from proteasomal...
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As mentioned above, I am having trouble with my subcellular fractionation of 4T1 cells. For my cytoplasmic lysis buffer, I am using 1% TritonX-100 Lysis buffer (20mM HEPES, 50mM NaCl, 1.5mM...
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