Law Cheng Lui Daniel

2 Questions 2 Answers 0 Followers

Questions related from Law Cheng Lui Daniel

HIF1a detection - Antibody not working?

Currently I am using HIF1a ab from CST (D5F3M) to probe for HIF1a levels. However, in my hypoxic samples I did not see any induction of HIF1a. I then attempted to rescue the HIF1a from proteasomal...

11 March 2020 1,214 0 View

Cytoplasmic lysis buffer (1% TritonX lysis buffer) is somehow lysing my nucleus and releasing my nuclear fraction into the cytoplasmic fraction?

As mentioned above, I am having trouble with my subcellular fractionation of 4T1 cells. For my cytoplasmic lysis buffer, I am using 1% TritonX-100 Lysis buffer (20mM HEPES, 50mM NaCl, 1.5mM...

06 January 2020 9,860 2 View