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Questions related from Lavinia Prosseda
Hi all, I am working with HPV pseudovirions and after some silver staining and ELISA experiments I realized I only have few micrograms of capsid protein (L1)/ml in my preps. Pseudovirions preps...
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Hi! What could be the reason why, after I add 1 volume of Trichloroacetic acid (TCA) to 4 volumes of my sample (an elution fraction from size exclusion chrom), I incubate 10 mins at 4°C and I...
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Hi, I did an overnight digestion using an His-tagged HRV-3C protease, my antigen of interest and cleavage buffer (containing 50 mM Tris-HCl pH 7.5, 150mM NaCl, 1 mM EDTA, 5mM DTT). The final...
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Can anyone address me to any protocol describing the best way to store my proteins (proteases) in glycerol and how to remove it before usage?
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