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Questions related from Lavinia Prosseda
Hi all, I am working with HPV pseudovirions and after some silver staining and ELISA experiments I realized I only have few micrograms of capsid protein (L1)/ml in my preps. Pseudovirions preps...
28 June 2023 8,625 0 View
Hi! What could be the reason why, after I add 1 volume of Trichloroacetic acid (TCA) to 4 volumes of my sample (an elution fraction from size exclusion chrom), I incubate 10 mins at 4°C and I...
02 August 2022 8,655 7 View
Hi, I have an heptamerized antigen of 259 kDa that I cleaved with a protease to induce the exposure of an epitope. However, I want to assess whether this cleavage induced the exposure or induced...
24 June 2022 8,257 1 View
Hi, I did an overnight digestion using an His-tagged HRV-3C protease, my antigen of interest and cleavage buffer (containing 50 mM Tris-HCl pH 7.5, 150mM NaCl, 1 mM EDTA, 5mM DTT). The final...
10 June 2022 6,453 1 View
Can anyone address me to any protocol describing the best way to store my proteins (proteases) in glycerol and how to remove it before usage?
07 May 2022 5,881 2 View
