Lavenniah Annadoray

2 Questions 4 Answers 0 Followers

Questions related from Lavenniah Annadoray

Can I compare the percentage of positively stained cells in my FACS data or do I have to multiply that by the total number of cells acquired?

I'm comparing cells from 3 genotypes and want to compare the percentage of positively stained cells. However, I've realised that just looking at the percentages alone do not take into account if...

22 September 2014 457 2 View

Could I use unstained cells to gate for negative cell population in flow cytometry?

I did not do FMOs as I did not have enough cells (using brain tissue here). Ive done isotype controls as well however they are have positive staining which could have possibly been due to an...

21 September 2014 7,161 6 View