Laura Zelenka

2 Questions 3 Answers 0 Followers

Questions related from Laura Zelenka

Why do astrocytes stick together after their dissociation from cell flasks?

Hello, I am isolating primary murine glial cells from different neonatal knockout strains (p0-p2). After several rounds (up to 4 times) of harvesting the microglia, I am dissociating the...

05 May 2019 9,903 4 View

How to get single astrocyte cells derived from neonatal mice and prevent cell clumping?

Hello, I am isolating primary murine glia cells from different neonatal knockout strains (P0-P4). I have issues getting single astrocyte cells after adding 4 ml of trypsin (2,5 %) to the PDL...

07 July 2018 5,991 8 View