Laura Díaz Marugán

4 Questions 11 Answers 0 Followers

Questions related from Laura Díaz Marugán

Separate serum from mouse blood sample

To obtain the serum from a mouse blood sample I perform this protocol: 1. Obtain the sample withouth any anticoagulant and transfer to a strile empty tube. 2. Leave the tube in a standing...

05 May 2017 9,472 4 View

Does anybody know how to stain paraffin brain samples with Cresyl violet ?

I have tried to stain paraffin brain sections with H&E staining but the result was an excessive pink sample (probably due to the eosin) and furthermore, I lost the majority of the samples...

08 August 2016 1,518 13 View

Has the anesthetic pentobarbital sodium any effect on the brain mouse?

We anesthetize the mice with pentobarbital sodium (50ul/25 mg), then we collect cerebrospinal fluid, and after bleed them we isolate the brain to study protein expression by western blot and and...

04 April 2016 4,397 2 View

Is there any way to count number and intensity of fluorescent points (they are proteins)?

I have marked a protein with a fluorescent antibody in a inmunohistochemistry sample and I want to count number and intensity of this protein, which looks like a point. I have tried the "analyze...

11 November 2015 9,654 12 View