Laura Llobet

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Questions related from Laura Llobet

Could Klentaq have residual 5'-3' exonuclease activity?

We are using a Klenow fragment like enzyme in qPCR assays with Taqman probes. Contrary to expectations, we obtain good amplification curves, despite the enzyme lacks 5'-3' exonuclease activity....

10 February 2023 7,655 0 View

How to perform non-truncated alignments in BLAST?

I would need to perform BLAST to provide individual blast results (non-truncated alignments) for some primers and probes with potential cross-reacting organisms. Specifically, I would need the...

25 March 2020 9,310 3 View

Do you have problems of contamination with RNase P?

I noticed that the RNAse P target for COVID-19 assay (CDC Atlanta) sometimes amplifies in NTC wells. Did you ever experience that? How should I proceed to avoid this contamination?

01 January 1970 9,767 1 View

Could Klentaq have residual 5'-3' exonuclease activity?

We are using a Klenow fragment like enzyme in qPCR assays with Taqman probes. Contrary to expectations, we obtain good amplification curves, despite the enzyme lacks 5'-3' exonuclease activity....

01 January 1970 3,005 3 View