Laura Guest

2 Questions 2 Answers 0 Followers

Questions related from Laura Guest

Why does my plasmid appear to get bigger after miniprepping?

This plasmid is quite old and has been stored at -20c since 2008. When I miniprep it from liquid cultures of about 5ml, it always runs slower on a gel, including the digested fragments. I also...

19 June 2023 8,948 2 View

Not seeing any pull-down of my protein of interest in Co-IP experiment, any ideas?

I am using a monoclonal antibody from Abcam for co-immunoprecipitation experiments, but it doesn't seem to be working. Abcam don't specify whether or not this Ab is suitable for IP experiments,...

07 October 2022 3,370 1 View