Laura Marise de Freitas

3 Questions 6 Answers 0 Followers

Questions related from Laura Marise de Freitas

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

25 February 2021 2,456 2 View

Does anyone know a protocol to quantify hydroxyl radicals and singlet oxygen formation in bacteria that DOES NOT involve microscopy?

I have been looking for a protocol to quantify ROS formation in bacteria using a spectrofluorimeter, NOT a fluorescence microscope, but I failed to find one. I have APF and SOSG (singlet oxygen...

11 January 2017 366 4 View

How to reduce the clump formation that one gets when scraping Porphyromonas gingivalis from agar plates?

We usually grow P.g and other anaerobes on agar plates and then scrap and suspend them into broth to obtain working suspensions. However, Pg is always very clumpy, and it is difficult to obtain...

21 June 2016 1,828 2 View