Lakshmi Vemulapalli

4 Questions 2 Answers 0 Followers

Questions related from Lakshmi Vemulapalli

3-D cell culture and normal mouse cell growth?

I am trying to grow 3D cultures from mouse xenografts (tumor inoculated ). I notice different cell phenotype (that looks different from rest of the culture) and wondering if I am growing normal...

24 December 2019 1,832 0 View

How to measure superoxides (O− 2) in biological samples?

Hello I am measuring the activities of catalase and SOD in my tissues homogeantes. I am wondering if we can measure superoxide levels in plasma or tissue lysates as it is converted to hydrogen...

09 March 2016 9,655 1 View

Why do house keeping genes not alter their expression under abnormal conditions?

I was told to look for house keeping genes like GAPDH or Beta Actin to quantify total target protein expression in tissues. I am just curious to know why such protein do not alter their...

28 July 2015 156 2 View

Why are primary antibodies preferred to incubate overnight in 4 degrees rather than room temperature?

Why antibodies binds specifically to their targets in 4 oC rather than room temperature. What exactly happens in this over night incubation?

27 July 2015 9,413 6 View