Lakshmi Vemulapalli

5 Questions 2 Answers 0 Followers

Questions related from Lakshmi Vemulapalli

3-D cell culture and normal mouse cell growth?

I am trying to grow 3D cultures from mouse xenografts (tumor inoculated ). I notice different cell phenotype (that looks different from rest of the culture) and wondering if I am growing normal...

24 December 2019 1,880 0 View

How to measure superoxides (O− 2) in biological samples?

Hello I am measuring the activities of catalase and SOD in my tissues homogeantes. I am wondering if we can measure superoxide levels in plasma or tissue lysates as it is converted to hydrogen...

09 March 2016 9,678 1 View

Why do house keeping genes not alter their expression under abnormal conditions?

I was told to look for house keeping genes like GAPDH or Beta Actin to quantify total target protein expression in tissues. I am just curious to know why such protein do not alter their...

28 July 2015 189 2 View

Why are primary antibodies preferred to incubate overnight in 4 degrees rather than room temperature?

Why antibodies binds specifically to their targets in 4 oC rather than room temperature. What exactly happens in this over night incubation?

27 July 2015 9,448 6 View

WNT-conditioned media for breast cancer organoids

My lab was using WNT conditioned media for human breast cancer organoids and we recently madea fresh batch of house made conditioned media. However, we do not have old batch to compare it with new...

01 January 1970 7,649 1 View