Kyle Potts

4 Questions 4 Answers 0 Followers

Questions related from Kyle Potts

Can I replace Invitrogen’s pLP1 + pLP2 + VSV-g plasmids with the pMDLg/pRRE + pRSV-Rev + pMD2.G 3rd gen packaging system off of Addgene?

We have a number of lentiviral plasmids from Eric Campeau (Addgene). Would I be able to use the pMDLg/pRRE + pRSV-Rev + pMD2.G 3rd generation packaging system that I can purchase off of Addgene in...

11 November 2014 10,084 1 View

What is the protocol for growing chemically competent cells?

I have a limited stock of Stbl3 chemically competent cells and would like to grow a stock that can be used for future experiments. Could some one recommend a protocol for growing chemically...

10 October 2014 9,241 9 View

Can anyone recommend plasmids to be used to generate lentiviruses to be used to make stable cell lines?

Can anyone recommend plasmids to be used to generate lentiviruses to be used to make stable cell lines? I have a few genes of interest I would like to clone into the expression vector and would...

07 July 2014 7,369 12 View

How can I determine if a mutation is homozygous or heterozygous?

I am planning to make some mutant p53 cell lines using the CRISPR system and homologous directed repair to introduce the mutant gene. What is the best way to determine if the mutant is homozygous...

04 April 2014 5,671 9 View