Kyal Anthony Young

4 Questions 7 Answers 0 Followers

Questions related from Kyal Anthony Young

What concentration of cells is the best to fix with Paraformaldehyde fixing ?

I have my protocol for fixing cells, however it tells me to fix at 2 million cells per ml with 2% PFA. I am wondering if i can use a higher concentration of cells to fix with as i normally have...

12 December 2014 6,368 3 View

Can anyone offer advice on flow cytometry base gating?

Hi all. Just wondering if I am gating correctly to lose doublets and debris but keep my cells. the attached image is of mouse peripheral blood analyzed on flowjo. when i look at images for...

12 December 2014 623 9 View

What is the best method for permeabilising cells?

Currently i have been using 90% ice cold methanol and incubating either on ice for 30 minutes or at -80 degrees for a few days. I need to permeabilise my cells for an intracellular phospho...

12 December 2014 8,273 7 View

Is anyone familiar with Human mononuclear and Granulocyte Panels?

I will be running a flow analysis of human peripheral blood with an interest in many cell subtypes. I have made some panels, can someone look over them and tell me if I will find the cells I am...

11 November 2014 1,614 7 View