Kunal Kumar

4 Questions 7 Answers 0 Followers

Questions related from Kunal Kumar

What is the protocol for Trichostatin A (TSA) treated cultured cell's HDAC activity assay?

I am working on cancer cell lines and want to check the effects of TSA on HDACs. Some article has suggested to treat the cell lysate with TSA and check the HDAC activity but we want to check the...

11 November 2018 1,505 1 View

Is there any protocol to check the effect of my drug on mitochondrial respiratory chain complex activity individually?

Hi all, I am working on cancer cells and my drug shows ROS production and mitochondrial membrane depolarization but I want to check the effect of my drug on mitochondrial respiratory chain complex...

03 March 2015 2,769 10 View

Why does the same dose of a drug produce a different effect in 60mm and 100mm plates of cancer cells?

When I seeded 0.1 million cancer cells in a 60mm plate and treated it with 3ul of fixed concentrations of a drug, it gave 40-50% cell death. But when we seeded with 0.6 million cells in 100mm...

03 March 2014 732 8 View

Where can I get full information regarding extra chromosome of AGS cell line from?

According to ATCC charactrization, AGS (Gastric Cancer) cell line have 49 chromosome and there is translocation between Chromosome 1 and Chromosome 8 but there is no description regarding any...

09 September 2013 581 0 View