Abhishek Kumar

3 Questions 4 Answers 0 Followers

Questions related from Abhishek Kumar

What are the methods to know the retrovirus positive transduced cells after infecting with retrovirus and antibiotic selection?

Hi I generated retrovirus by transfecting the plasmid with my gene of interest in packaging cell line and checked my gene in generated retrovirus by RT-PCR. When I infecting HEK 293 cell line with...

02 February 2019 2,645 3 View

Did anyone suggest, whether I could use the HCT116 cell line for MICA down regulation after infecting Adenovirus type 41?

Hi I want to use HCT116 cell line to check the down regulation of MICA/MICB surface expression level after infecting with Adenovirus type 41. For this cell should have surface expression of...

10 October 2018 1,189 3 View

Can I get the explanation about non-specific binding of "Protein" with "Protein G sepharose bead" in absence of IP-antibody?

During performing Co-IP with two purified proteins. As a IP-antibody negative control, I incubated either of these protein with protein G-sepharose bead without IP-antibody for 1:30 hrs and washed...

08 August 2018 5,104 3 View