Kshitiz Raj Shrestha

3 Questions 4 Answers 0 Followers

Questions related from Kshitiz Raj Shrestha

How can LIVE / DEAD staining of stem cells on electrospinned nanofiber be performed?

I wanted to perform LIVE /DEAD staining of Adipose derived Stem Cells (ASC) on the electrospinned Poly-L-lactide co-e-caprlatone(PLCL) nanofibers. 1 m2 PLCL nanofiber (collagen coated) was placed...

30 June 2014 8,098 3 View

Human bone marrow derived Mesenchymal stem cell expansion?

To what passage number can human bone marrow derived from Mesenchymal stem cells be expanded for differentiation studies?

11 October 2013 3,557 3 View

Need help with VEGF PLGA nanoparticles

I am currently working with VEGF PLGA nanoparticles for lymphedema treatment. I am using PVA 1% as a stabilizer and dissolving 0.1gm PLGA in 4ml DCM. I am following multi emulsion (w1/o/w2) by...

18 June 2013 1,388 2 View