Kseniia Bondarenko

3 Questions 5 Answers 0 Followers

Questions related from Kseniia Bondarenko

Why in triple staining antibody does not work, while in double staining - does?

Hi! My question is how to troubleshoot the triple immunohistochemical staining. I have two well-validated antibodies, stained with Alexa Fluor 488 and 546, respectively. Together, they work very...

06 June 2019 5,495 4 View

Is Trifluoroacetic acid (TFA) toxic to neurons?

The compound I am ordering is offered as the Trifluoroacetic Acid Salt. I will use 25-100 uM concentrations of it, and in literature, I cannot find the toxicity of TFA to brain slices or cell...

05 May 2018 9,298 4 View

Where to start when determining the key regulator of energy availability during synaptic transmission?

Since I am very new to this field, I kindly ask you to help me with determining the start points, from which I can push off for a further methodology development. If you were faced these tasks,...

01 January 2017 4,860 5 View