8 Questions 19 Answers 0 Followers
Questions related from Ks Lim
I am currently culturing the T-CLL, Kit-225 cell line. My culture condition is RPMI 10% FCS with 20ng/ml IL2 (R&D, about 420IU/ml). Cells look viable but proliferation rate is slow. Is there...
28 June 2017 1,977 6 View
I am facing problem in qPCR analysis. The file attached shows the melt curve of GAPDH. In my setting, my template was 100ng and primer concentration 500nM in 20ul. Cycling number was 40 cycles. In...
02 January 2016 8,095 4 View
I am currently comparing ULBP-4 expression in cancer cells. The method I used for qPCR as follow: a) cDNA synthesis = 1ug of RNA in 20ul b) I diluted the cDNA 10X prior to qPCR c) I used 4ul of...
31 October 2015 4,047 8 View
A standard protocol for intracellular staining usually separate the fixation and permeabilization part. Can I do the fixation and permeabilization at one time by incubating cells with 3.7%...
17 July 2014 8,581 5 View
Some protocols mention primary antibodies should be diluted in 5%BSA whereas some protocols mention primary antibodies should be diluted in 5% non-fat milk. What is the difference? What is the...
26 May 2014 8,167 19 View
Does anyone know how to reduce cell clumping after cells are treated with enzyme-free dissociation buffer? Is autoMACs rinsing solution (contains PBS and EDTA) helpful to prevent cell clumping in...
20 March 2014 8,055 8 View
see above
17 March 2014 6,048 0 View
Currently, I am doing a research on NKG2D ligand expression on cancer cells. I would like to use dual color staining, which are APC-conjugated Ab for ULBP-1 and PE-conjugated Ab for ulbp-2....
04 March 2014 5,926 19 View
