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Questions related from Krupa Mysore
Hello, Has anyone had luck measuring LPS from human plasma? Which ELISa kit do you recommend? I am getting a lot of interference from color of the plasma. Thanks
16 November 2020 6,784 1 View
I am trying to look at proliferation and cytokine (IFN-g) production on PBMC's with and without blocking antibodies. Trying to demonstrate that there is a difference in T cell function with and...
15 June 2017 2,353 5 View
Hello all, I want to use Ki67 along with IFN, TNF in relation to T cell inhibitors like PD-1. If I use PBMC's and stim with PMA/iono for 6 hours with golgi inhibitors I am unable to see Ki67...
28 February 2017 6,248 4 View
In human T cell studies with PBMC's what are the typical T cell activation markers that people have successfully used - What works in unstimulated and a 6 hour PMA or SEB stim condition?
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Hello immunology experts! I am working with human PBMC's and doing T cell studies. Wondering what is the typical percentage of cells that you lose after overnight resting before...
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Hello, I am stimulating T cells for a study. When I do my cell count with Tryphan blue ( 10 micro liter of tryphan blue and 10 microliter of cell suspension); do you multiply the cell count by 2?...
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