Krupa Mysore

5 Questions 5 Answers 0 Followers

Questions related from Krupa Mysore

Experience measuring human LPS in plasma?

Hello, Has anyone had luck measuring LPS from human plasma? Which ELISa kit do you recommend? I am getting a lot of interference from color of the plasma. Thanks

16 November 2020 6,691 1 View

Can I simultaneously do CFSE proliferation and IFN-cytokine measurement on PBMCs

I am trying to look at proliferation and cytokine (IFN-g) production on PBMC's with and without blocking antibodies. Trying to demonstrate that there is a difference in T cell function with and...

15 June 2017 2,314 5 View

Problems with Ki67 staining to look at T cell activation/proliferation

Hello all, I want to use Ki67 along with IFN, TNF in relation to T cell inhibitors like PD-1. If I use PBMC's and stim with PMA/iono for 6 hours with golgi inhibitors I am unable to see Ki67...

28 February 2017 6,157 4 View

Loss of cells after resting PBMCs overnight?

Hello immunology experts! I am working with human PBMC's and doing T cell studies. Wondering what is the typical percentage of cells that you lose after overnight resting before...

11 November 2015 9,939 7 View

Any advice on Trypan blue during cell counts?

Hello, I am stimulating T cells for a study. When I do my cell count with Tryphan blue ( 10 micro liter of tryphan blue and 10 microliter of cell suspension); do you multiply the cell count by 2?...

21 October 2015 9,123 2 View