Kristy Shimp

3 Questions 2 Answers 0 Followers

Questions related from Kristy Shimp

Does anyone know where I could procure a sample of recombinant D1 and/or D2 receptor protein (or a stable cell line over-expressing one of these)?

I am using an Abcam (ab20066) anti-D1 antibody as well as a Millipore (AB5084P) anti-D2 antibody for Western blotting of PFC and striatum membrane fraction homogenates. Unfortunately, there is a...

14 June 2012 6,625 6 View

Has anyone found reliable D3 and D4 dopamine receptor antibodies?

I'm looking to do some Western's and possible ELISA, IHC, or IF and am leery of ordering an antibody that has no references or that no one has recommended. I've found a couple of products online,...

12 April 2012 7,700 1 View

What is the best method for dissecting anatomically specific rat brain tissue?

I'm trying to isolate striatum, PFC, and amygdala from whole brain tissue from Long-Evans rats in a uniform manner (for use in a ChIP and/or immunoblot procedure(s)). I've tried doing this...

10 April 2012 801 21 View