Kristy Shimp

5 Questions 2 Answers 0 Followers

Questions related from Kristy Shimp

Can anyone recommend a resource for tritium microscales to use in an autoradiography experiment?

I am preparing to run an autorad experiment using D1 and D2 tritiated ligands; however, it recently came to my attention that GE no longer makes the tritium microscales and the only other...

10 July 2013 6,698 2 View

Has anyone used perfused brain tissue for a ChIP assay?

I am planning on doing a ChIP of rat nucleus accumbens tissue using the Magna ChIP G Tissue Kit. The protocol calls for a whole punch on 300 um sections. I sliced a non-perfused brain at this...

28 January 2013 4,623 3 View

Does anyone know where I could procure a sample of recombinant D1 and/or D2 receptor protein (or a stable cell line over-expressing one of these)?

I am using an Abcam (ab20066) anti-D1 antibody as well as a Millipore (AB5084P) anti-D2 antibody for Western blotting of PFC and striatum membrane fraction homogenates. Unfortunately, there is a...

14 June 2012 6,653 6 View

Has anyone found reliable D3 and D4 dopamine receptor antibodies?

I'm looking to do some Western's and possible ELISA, IHC, or IF and am leery of ordering an antibody that has no references or that no one has recommended. I've found a couple of products online,...

12 April 2012 7,724 1 View

What is the best method for dissecting anatomically specific rat brain tissue?

I'm trying to isolate striatum, PFC, and amygdala from whole brain tissue from Long-Evans rats in a uniform manner (for use in a ChIP and/or immunoblot procedure(s)). I've tried doing this...

10 April 2012 826 21 View