Can anyone help me to optimize QQQ parameters for Dansyl-Estradiol...523...251?

I (attempted to) dansylate 100ng/mL d5-Estradiol using 200uL 100mM NaHCO3, pH=10.5, and 100uL of 1mg/mL dansyl chloride in Acetonitrile, incubation time = 20min, temp= 31.1C (targeted 30C). I am...

09 January 2015 8,232 1 View