Kristie Leigh Smith

5 Questions 3 Answers 0 Followers

Questions related from Kristie Leigh Smith

Leica Aperio AT2 not detecting blank negative control stained tissue... tips on imaging?

Our Leica Aperio AT2 is not able to detect our negative control tissue tissue, even with 'faintly stained' settings selected. We have spoken to customer service but they do not have fixes...

10 June 2021 3,216 2 View

Factors that modulate the expression of Iba-1 in microglia?

Several papers talk about states (stressors) and pathological damage altering microglial behaviour and morphology and, as such, Iba-1 expression. However I'm struggling to find in the literature...

06 June 2018 8,163 3 View

How to strip fluorescent antibody staining from tissue?

Does anyone have a working protocol or an idea of products I could use in the lab to strip fluorescent antibodies from fixed mouse brain tissue? I want to restain the same tissue with a Nissl stain.

12 October 2016 7,348 4 View

Making own Cresyl Violet/Nissl (or similar stain) in the lab?

Hi all, I was just wondering whether it is possible to make Cresyl Violet/Nissl (or a similar stain to determine location of implanted electrode) in the lab instead of buying it? I have a...

26 July 2016 5,650 2 View

How to choose the right wireless chronic electrode for in vivo recordings in freely moving mice?

I am looking for a set of wireless electrodes to chronically implant into the amygdala of my freely moving experimental mice. I have a few wireless electrodes from Neuronexus, however the company...

30 June 2016 2,867 1 View