Kristie Leigh Smith

11 Questions 7 Answers 0 Followers

Questions related from Kristie Leigh Smith

Leica Aperio AT2 not detecting blank negative control stained tissue... tips on imaging?

Our Leica Aperio AT2 is not able to detect our negative control tissue tissue, even with 'faintly stained' settings selected. We have spoken to customer service but they do not have fixes...

10 June 2021 3,273 2 View

Factors that modulate the expression of Iba-1 in microglia?

Several papers talk about states (stressors) and pathological damage altering microglial behaviour and morphology and, as such, Iba-1 expression. However I'm struggling to find in the literature...

06 June 2018 8,265 3 View

Microglial morphology during apoptosis - beyond the cell body?

Current literature states that during apoptosis of microglial cells, the cell body can decrease in size, membrane blebbing can occur, and measures such as nuclear condensation, fragmentation can...

07 November 2017 8,337 2 View

Will Annexin V tag apoptotic microglia?

I am trying to determine whether microglia are undergoing apoptosis in an experiment. I tried Active Caspase 3 was unable to get the antibody to work (IF).I was considering a different apoptotic...

29 August 2017 4,765 4 View

How to strip fluorescent antibody staining from tissue?

Does anyone have a working protocol or an idea of products I could use in the lab to strip fluorescent antibodies from fixed mouse brain tissue? I want to restain the same tissue with a Nissl stain.

12 October 2016 7,430 4 View

Making own Cresyl Violet/Nissl (or similar stain) in the lab?

Hi all, I was just wondering whether it is possible to make Cresyl Violet/Nissl (or a similar stain to determine location of implanted electrode) in the lab instead of buying it? I have a...

26 July 2016 5,721 2 View

Does anyone know the Emax of WIN55,212 - 2 in rodents (specifically mice)?

Continuing on from a previously posted question where the half-life of WIN55,212 - 2 was discussed, (see:...

19 July 2016 4,494 3 View

How to choose the right wireless chronic electrode for in vivo recordings in freely moving mice?

I am looking for a set of wireless electrodes to chronically implant into the amygdala of my freely moving experimental mice. I have a few wireless electrodes from Neuronexus, however the company...

30 June 2016 2,930 1 View

Why is my IHC microglia stained tissue getting what appears to be oxygen damage after mounting with Entellan?

We have been using an IHC microglia stain on some mouse brain tissue after some experiments we ran.We followed our labs proven protocol for staining, and we dehydrate in graded ethanol and finally...

20 April 2015 8,224 4 View

What would be a suitable protein to stain for in comparing altered dendritic morphology of stressed compared to control mice?

We have cleared brains of lipid using the Clarity method and are now preparing to stain our tissue. A focus of mine is comparing dendritic/spine retraction in stressed mice to that of control...

03 March 2014 1,836 0 View

Would allowing patients to use electronic equipment confound results of a melatonin/cortisol sleep protocol?

Controlling for and monitoring light, food and body temperature, as well as monitoring with EEG, EMG, ECG, and taking saliva samples of melatonin and cortisol, would allowing patients to use their...

04 October 2013 3,726 4 View