Krister Stokke

4 Questions 5 Answers 0 Followers

Questions related from Krister Stokke

Has anyone made a BRAF resistant melanoma cell line?

I was wondering if anyone has any experience with making a BRAF resistant melanoma cell line. Preferably Dabrafenib or Vemurafenib resistance. If so, how many passages did i take and what cell...

04 April 2018 5,958 4 View

Does anyone have any experience using an incucyte zoom to find IC50 values?

Im trying to determin the IC50 values in a melanoma cell line on different drugs. Does anyone have any experience using the inculcyte zoom for this? My option is using a resazurin essay, but I'm...

02 February 2017 3,008 1 View

When generating lentivirus, has anyone got any experience with trypsinizing the 293T cells after harvest?

Instead of seeding say 10 x 100mm dishes is it possible to just seed one dish with 293t cells and then transfer the transfected cells into a bigger bottle or a new environment?

02 February 2016 4,291 3 View

Does any one have a good protocol/procedure for a permanent venous catheter in mice for drug administration, which allows free movement for the mice?

We are researching the effects on different drugs inhibiting brain metastases in mice. So far drugs have been administered through the tail vain, but this sets limitations to how often one can...

01 January 1970 7,230 2 View