Krishnagaanth Mohan Kumar

2 Questions 3 Answers 0 Followers

Questions related from Krishnagaanth Mohan Kumar

How to remove LPS from the E.coli expressed proteins for downstream work on immunecells ?

I have tried polymixin B beads from thermo (Detoxi-Gel™ Endotoxin Removing Gel). The purified protein were incubated with beads for 1 hrs in 4C, rotation.i have used protein to bead ratio of...

05 August 2025 8,093 0 View

How to purify Protein from Pichia X-33 cells using ammonium sulphate precipitation methods?

I am trying to purify a heterologous protein with His tag in yeast but I am facing issues with binding my protein to Ni-NTA beads. Please suggest some methods to purify my protein of...

11 April 2023 808 7 View