Krishna Kant Gupta

4 Questions 3 Answers 0 Followers

Questions related from Krishna Kant Gupta

I have one strep tagged protein. The protein is not eluting upto 10mM desthiobiotin. What should I do?

The elution buffer is 100 mM Tris(pH=8.0) 150 mM NaCl 1mM EDTA (This is not a metalloprotein) I have adjusted the pH to 8.0. I also have loaded 2 microlitre beads which clearly shows my...

25 April 2025 7,801 2 View

Membrane simulation -reg

Dear all, How to simulate small molecules inside biological membrane? I have an expertise in gromacs but quite not sure about membrane simulation. Please suggest a good tutorial on...

01 January 1970 5,739 2 View

How to retrieve PSSM matrix from PSSM file (in ASN format) received from NCBI PSI-BLAST?

I have PSSM file in ASN format. I need to retrieve the numeric values from matric. How to do that?

01 January 1970 4,651 0 View

Protein protein flexible blind docking

I want to do protein protein flexible blind docking ..Please let me know how to do it?

01 January 1970 2,799 1 View