Krishan Selwal

4 Questions 12 Answers 0 Followers

Questions related from Krishan Selwal

How to prepare mucin containing medium?

Hi, I am working on mucin utilizing microorganisms and for this I need to prepare a medium for the same. I have encountered with the following a) Using porcine stomach (type II) mucin, is there...

21 August 2018 8,764 11 View

How can I prevent smeared spots in paper chromatography?

I am trying to analyse starch hydrolyzed products by paper chromatography and performed with standard and unknown sample. But after I noticed dark smeared all the way on the paper blot for the all...

20 October 2015 5,010 4 View

What is suitable protocol for FITC antibody immunofluorescence in yeast?

Hi All, I am facing difficulty with immunofluorescence of yeast with FITC antibody which supposedly bind with V5 epitope present on the cell surface. I have tried three times with (invitrogen V5...

26 September 2015 7,345 7 View

How can I overexpress protein in yeast for surface display?

well, I have transformed yeast with vector containing gene of interest and it is showing activity on plate. But facing difficulty when tried western blot and SDS PAGE. Its little frustrating that...

02 September 2015 7,225 3 View