Kotwal Shifa Bushra

7 Questions 19 Answers 0 Followers

Questions related from Kotwal Shifa Bushra

Can one guide RNA for Cpf1 in CRISPR-Cas12a sufficient to achieve target mutation in bacteria ?

Hello, So my query is for knocking down a gene. Will one guide serves the purpose, or does it require more than one guide RNAs in the CRISPR-Cas12a system in bacteria?

21 July 2022 9,409 7 View

Troubleshooting with false positives in the ligation mix? What can be the reason and what to do to avoid false positive clones?

Hey, I am facing the a problem in ligation transformation. my clones for both 1:3 and 1:6 molar ratio ligation mix giving false positives, I am not understanding what might be the problem for...

05 May 2021 3,168 7 View

Troubleshooting for Ligation transformation?

I am working on molecular biology and vaccine technology. I am repeatedly facing a issue in Ligation transformation for which I have tried troubleshooting it by many ways. By increasing or...

11 April 2021 3,865 10 View

Can I purify my PCR product without gel extraction by PCR cleanup? If yes, What is the percentage of yield I get by PCR cleanup?

I am planning to do a cloning with PCR product i.e., my insert for cloning. I have observed 50% loss of yield from gel extraction of my PCR product. I have no non-specifics in my PCR product. I...

30 March 2021 1,207 23 View

Yield of Plasmid is low in Maxipreparation of plasmid for 200ml? What might be the reasons for it?

Hi, I am working with a plasmid which is known to have very low copy number and when I am isolating it by midiprep alkaline lysis method, I am getting good amount of plasmid i.e., 16.56ng/ul....

12 December 2020 1,509 3 View

To study antioxidant profile which would be better to analyse ,Serum or Tissue from mice?

I am working on toxicity induced by drugs on invivo model(mice) and few compounds that have antioxidant properties that would be used to reduce this toxicity. The organs I would be looking for...

23 March 2020 4,244 6 View

how to purify and quantitate nanoparticles once synthesized for use in invivo studies?

I am working on nanoparticles and its biological applications . My question is once nanoparticles are synthesized by what methods can they be purified to eliminate contaminants,impurities present...

28 February 2020 2,180 5 View