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Questions related from Kotwal Shifa Bushra
Hello, So my query is for knocking down a gene. Will one guide serves the purpose, or does it require more than one guide RNAs in the CRISPR-Cas12a system in bacteria?
21 July 2022 9,403 7 View
Hey, I am facing the a problem in ligation transformation. my clones for both 1:3 and 1:6 molar ratio ligation mix giving false positives, I am not understanding what might be the problem for...
05 May 2021 3,167 7 View
I am working on molecular biology and vaccine technology. I am repeatedly facing a issue in Ligation transformation for which I have tried troubleshooting it by many ways. By increasing or...
11 April 2021 3,863 10 View
I am planning to do a cloning with PCR product i.e., my insert for cloning. I have observed 50% loss of yield from gel extraction of my PCR product. I have no non-specifics in my PCR product. I...
30 March 2021 1,203 23 View
Hi, I am working with a plasmid which is known to have very low copy number and when I am isolating it by midiprep alkaline lysis method, I am getting good amount of plasmid i.e., 16.56ng/ul....
12 December 2020 1,505 3 View
I am working on toxicity induced by drugs on invivo model(mice) and few compounds that have antioxidant properties that would be used to reduce this toxicity. The organs I would be looking for...
23 March 2020 4,240 6 View
I am working on nanoparticles and its biological applications . My question is once nanoparticles are synthesized by what methods can they be purified to eliminate contaminants,impurities present...
28 February 2020 2,178 5 View