Kk Chan

3 Questions 7 Answers 0 Followers

Questions related from Kk Chan

How can I assess the quality of DNA extracted from FFPE tissues?

I have extracted DNA from FFPE tissues (7 - 9 years ago) using Qiagen FFPE kits. I measured the DNA concentration by nanodrop and loaded ~16-20ng DNA to 1.5% gel, but it seems to me there's...

10 October 2014 2,363 4 View

Can anyone help with human neutrophil extraction?

I want to isolate human neutrophil after getting the PBMC first. I first put the diluted blood (2ml blood with 2 ml PBS) onto the ficoll paque to extract PBMC. The I took out the bottom red...

29 April 2014 4,282 10 View

Can anyone help with cell clumps after Ficoll PBMC extraction?

I carried out PBMC extraction using Ficoll paque PLUS. However after using PBS washed for twice, cells clump together and cannot be resuspended. Could anyone help to see what the problem is? Pic...

13 March 2014 1,126 14 View