Kitae Kim

3 Questions 1 Answers 0 Followers

Questions related from Kitae Kim

Vertical protein band in SDS-PAGE?

After immunoprecipitation with specific antibody and Protein G, samples were eluted with elution buffer, SDS sample buffer, and reducing agent at 70 °C for 5 minutes. Also, samples were incubated...

06 July 2023 8,936 3 View

Can 3xFLAG peptide (MDYKDHDGDYKDHDIDYKDDDDK) be detected by western blot?

I'm expressing 3XFLAG peptide (MDYKDHDGDYKDHDIDYKDDDDK, ~2.9 kda) using in vitro translation system and trying to detect this peptide using western blot (1st Ab = Monoclonal ANTI-FLAG® M2 antibody...

07 June 2021 7,908 2 View

Inaccurate molecular weight in SDS-PAGE?

I ordered a synthetic peptide (sequence = MHHHHHH) This peptide has a molecular weight of 0.97 kDa based on a web-based prediction tool and mass spectrometry. However, when I ran it on the...

21 January 2021 3,579 4 View