Kitae Kim

5 Questions 1 Answers 0 Followers

Questions related from Kitae Kim

Questions about liposome preparation and membrane protein reconstitution?

Hi, I'm doing experiments reconstituting membrane proteins to liposome. And have a few questions. 1. If I use buffer while hydration of lipid, buffer can encapsulated into liposome. Then,...

11 March 2024 1,842 1 View

Vertical protein band in SDS-PAGE?

After immunoprecipitation with specific antibody and Protein G, samples were eluted with elution buffer, SDS sample buffer, and reducing agent at 70 °C for 5 minutes. Also, samples were incubated...

06 July 2023 8,988 3 View

Can 3xFLAG peptide (MDYKDHDGDYKDHDIDYKDDDDK) be detected by western blot?

I'm expressing 3XFLAG peptide (MDYKDHDGDYKDHDIDYKDDDDK, ~2.9 kda) using in vitro translation system and trying to detect this peptide using western blot (1st Ab = Monoclonal ANTI-FLAG® M2 antibody...

07 June 2021 7,955 2 View

Inaccurate molecular weight in SDS-PAGE?

I ordered a synthetic peptide (sequence = MHHHHHH) This peptide has a molecular weight of 0.97 kDa based on a web-based prediction tool and mass spectrometry. However, when I ran it on the...

21 January 2021 3,722 4 View

PBAD promoter activity in lactose rich condition in E. coli?

Lactose is a combination of one galactose and glucose molecule. E. coli can degrade lactose into galactose and glucose by LacZ. If the cells are in the glucose-rich condition, the pBAD promoter...

13 March 2020 4,194 0 View