Kinjal V Parikh

5 Questions 5 Answers 0 Followers

Questions related from Kinjal V Parikh

You receive a paraffin embedded specimen for HPV typing, which method should you use? PCR? RFLP? Hybrid-capture? Invader?

All tech. can be used for HPV But don't know which one is for embedded tissue

05 May 2018 7,069 4 View

Can we store DNA in alcohol for long period of time?

I am using TE Buffer for DNA storage.

07 July 2014 2,490 7 View

Why is cloning required before sequencing?

I am working on barcoding of zoanthids. And I want to do sequencing of COI and ITS-rDNA. I have amplified both the genes. Now what should be the next step to get sequence of both the genes?

04 April 2014 7,641 12 View

What is the best kit for PCR Product Purification? Is there any manual method available for purification?

I have amplified COI gene and for further sequencing, I need to purify it. Right now, I am looking for a purification kit. can anyone suggest one?

03 March 2014 526 8 View

What is the best technique to isolate DNA from marine animals?

I have tried lysis buffer with Proteinase K but I am not getting a 100% intact band of DNA.

03 March 2014 4,842 4 View