Khurshed Akabirov

3 Questions 1 Answers 0 Followers

Questions related from Khurshed Akabirov

How to hybridize short probes (27-57 nt) to CAS9 mRNA?

Hello, I purchased short DNA Oligo probes containing 27-57 nucleotides . I am trying to hybridize those probes into my CAS9 mRNA to its multiple locations. I guess I need to use mRNA-ISH method...

09 September 2021 8,618 0 View

What are some important techniques for protein induction and SDS-PAGE analysis?

Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...

04 March 2021 8,508 3 View

What is the difference between PCR and the initial steps (before SDS gel) of DNA sequencing? Which method is more effective?

I apologize if my question is not clear. I have a pure chemistry background and I am very new in a Protein Chemistry and Enzymology science.

26 September 2020 8,168 5 View