Khem Chand Saini

8 Questions 18 Answers 0 Followers

Questions related from Khem Chand Saini

How to Sequence cultured Cyanobacteria (most probably Nostoc spp.) Isolated from lichens?

Hello folks, I have amplified 4 cultured filamentous cyanobacteria isolated from lichens using Cyano 106F and Cyano 781 R primers. PCR band was very good. Then i go for sequencing using same...

02 May 2019 3,455 3 View

How can reduce multiple band/double band problem in PCR amplification?

I am amplified DNA of algae by using ITS universal primer. In some PCR reaction i got double band, how can i reduce it?

19 August 2018 4,363 14 View

Which methods used for the fixation of unicellular algal samples for SEM?

Among Osmium tetroxide and glutaraldehyde, which one is more suitable for fixing of unicellular algae for SEM. Please provide me protocol if anyone having?

30 April 2018 5,219 3 View

Which dye is most suitable for nucleus staining of unicellular algae?

I want to microscopic studies of cultured unicellular algal cells. I want to know which dye is most widely used for nucleus staining for algae.

30 April 2018 5,114 8 View

For unicellular algal broth culture what is optimum condition like temprature, Light and Shaking speed (RPM?) for faster growth?

I am using 3N BBM broth media in a 250 ml flask for unicellular green algae cultivation. Which condition is very good for faster algal growth? How much days will take for good growth?

03 April 2018 7,014 3 View

Which media is used for trentepholia suspension culture?

I want to culture Trentepholia and Printzina. Which media is best for faster growth? How long time will take for getting good amount of biomass?

03 April 2018 7,517 2 View

Without using formaldehyde, does anyone have any ideas how to preserve cultured unicellular algal samples for a morphological and molecular studies?

I am isolates Photobionts from lichens and cultured them. I am getting sufficient biomass on agar plate containing 3N BBM media and broth as well. How can i preserve this biomass for future studies?

23 March 2018 8,397 7 View

Why soil extract is important component for algal culture media?

why soil extract is important component for algal culture media?

31 May 2017 7,719 1 View