Khairul Amran

5 Questions 11 Answers 0 Followers

Questions related from Khairul Amran

What is the expression of Bax and Bcl-2 protein in normal state?

Is it normal when a Bcl-2 protein is highly expressed compared to Bax protein in normal state (control group)? This pattern also appeared in an apoptosis-induced group with higher expression...

06 June 2014 2,545 9 View

Which one do you recommend, either incubate the primary antibody in TBST or in blocking buffer?

Incubation with primary antibody Which is recommended, either incubate the primary antibody in TBST or in blocking buffer? Any suggestion? Thank you.

05 May 2014 3,581 12 View

Did the “stripping and reprobing” cause loss of targeted protein?

I stipped and reprobed my PVDF membrane 4 times using 3 different antibodies (bax, bcl-2 and p53) and loading control (GAPDH), then I stripped and reprobed the same membrane with GAPDH again (as...

05 May 2014 1,124 6 View

Any suggestions on a method/statistical analysis to evaluate protein expression by western blotting?

I am very new in this kind of research (western blotting). I want to study the protein expression in mice in three difference groups (n=6). How should I set up my methods and statistical analysis...

05 May 2014 4,630 4 View

What is the suitable percentage of acylamide gel needed for SDS-PAGE electrophoresis?

I am very new to western blotting. I'd like to analyze the expression pattern of my protein of interest in liver and lung tissues (20-60 kDa). Can anyone tell me the suitable percentage of...

04 April 2014 1,308 8 View