Kevin Masterson

4 Questions 2 Answers 0 Followers

Questions related from Kevin Masterson

When gowing biofilms in vitro, why is the media changed following the initial attachment?

Hi all, I've seen in a number of publications involving the growth of biofilms in microtitre plates, that the media is changed following an initial attachment phase (which can range from 2 - 8...

02 September 2020 7,085 3 View

Effects of TC treated and non-TC treated 96-well plates with bacterial strains?

I'm performing assays on a number of bacterial strains, some of which are capable of forming biofilms. What effects would a "tissue culture treated" plate and a "non-tissue culture treated" plate...

28 January 2019 6,628 5 View

What can cause lower absorbance readings from center of 96-well plate following MTT?

I have been running drug treatment assays on cell lines in 96-well plates. Following 72 hours incubation, I perform a MTT assay to assess cell viability following the treatment. I am frequently...

15 May 2018 8,828 4 View

How to prevent Silver Nitrate (AgNO3) precipitating in growth broth?

I am trying to determine the MIC of Silver Nitrate (AgNO3) against a number of bacterial strains using broth micro/macro dilutions method. I prepared the AgNO3 in distilled water to a...

24 January 2018 7,657 7 View