Kelly McGlynn

3 Questions 6 Answers 0 Followers

Questions related from Kelly McGlynn

Leave GFP uncompensated during flow cytometry?

I'm doing a differentiation experiment with flow analysis, comparing two cell lines: one is the wt cell line, and the other is a stable line transduced with our GOI + GFP marker. The GFP is just a...

08 August 2017 4,155 4 View

Stable cell line infected with gene-BFP, BFP visible but no protein on WB?

I infected MEL cells with lentivirus encoding my protein of interest (Flag tagged) with a BFP marker. So the construct goes EF1a promoter-gene-Flag, PGK-BFP-puro. I selected the cells with...

03 March 2017 5,028 2 View

Power calculation for colony formation assay?

Hi, Does anyone know how to do a power calculation for a bone marrow colony formation (serial replating) assay? I'd be looking for a difference in mean number of colonies on the 3rd replating...

04 April 2016 8,651 2 View