Keerti Potluri

3 Questions 5 Answers 0 Followers

Questions related from Keerti Potluri

What is the best method to test dilutions for transfection of HeLa cells?

Hi all, I am trying to get the best dilution which gives me 80% confluency next day for transfecting HeLa cells. I supposed to get cell count 1.7X times the next day (which means if I am seeding...

04 April 2017 8,044 4 View

Trying to troubleshoot the Western Blot for my Hela cells stably transfected with GFP and with my protein of interest TDO2 with HA tag?

Hi all. I have western blotting twice to see the expression of GFP in HeLa cells, but unfortunately both times I saw band for GFP at ~ 44kDa ( we expect at ~25kDa) and a band for TDO2 protein...

03 March 2017 2,881 3 View

When is the best time to expand HEK 293 cells?

Hi everyone, I am new to cell culture and I am trying to maintain HEK 293 cells. The cells are 100% confluent and so my supervisor told me to passage , expand and freeze them as I want. I passaged...

03 March 2017 5,088 6 View