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Questions related from Keerthy Vijayan
While working with DNA polymorphism analysis in DNAsp, I found some sequences were omitted from the analysis due to the presence of gaps (deletions) in some of the sequences as well as insertions....
02 April 2018 3,937 1 View
After many trial and errors I got a band in my PCR amplification. I have used taq, DNTP mix from the same company (Termo scientific). I think it could have helped me. But now the problem is the...
28 May 2016 8,024 7 View
while isolating DNA from animal tissue I usually get bands in the upper region of the gel. But today after DNA isolation AGE was done at 100v for 30 minutes and the gel documentation yielded the...
19 February 2016 8,045 4 View
while doing the DNA isolation of a sample, using DNAzol reagent, I got a very straight band of DNA. Is it a DNA band or something else? The picture is attached along with.
15 February 2016 5,611 8 View
Again while doing the PCR amplification of snail DNA, I got stuck with no visible bands on gel. But there is the presence of primer dimers in the gel. The DNA pellet I got readily dissolved in...
07 February 2016 6,234 5 View
While doing the PCR amplification of 16s rRNA gene of snail samples, I got a very nice pellet of DNA which dissolved in water readily. But when the PCR amplification was performed there appeared...
29 January 2016 3,206 6 View
Iam doing research on the molecular phylogeography of the giant african snail in India. How can I calculate the mutation rate/time (time from which the change has occurred in the sequence)? Is...
25 January 2016 9,902 6 View
We have done methanol extraction of a plant sample using a soxhlet apparatus. In order to get rid of the contents of methanol from the extract what procedure has to do? Simply evaporating the...
27 November 2014 2,539 4 View
