Keerthana Sekar

4 Questions 6 Answers 0 Followers

Questions related from Keerthana Sekar

What is the co-relation between NO production and phagocytosis by macrophages on influenza infection?

I am trying to measure the Nitric oxide production by RAW 264.7 macrophage on infection with influenza virus using NO assay. I infect the cells for 5 hrs and measure NO at 12hrs and 24hrs....

13 October 2014 5,852 4 View

Will addition of LPS and IFNg to the RAW 264.7 culture stress the cells resulting in poor or no phagocytosis of influenza virus?

Addition of both LPS and IFNg stimulates the macrophages. Upon infection and stimulation of macrophages with LPS and IFNg, will the cells get stressed that one could not observe the infection of...

05 August 2014 9,695 2 View

Can anyone suggest me a method to detect phagocytosis by Raw 264.7 on infection with influenza virus?

I would like to know an experiment to detect phagocytosis by raw 264.7 cells on infection with influenza virus. I used Vybrant phagocytosis assy kit and do not see any phagocytosis both in cells...

28 July 2014 8,421 5 View

Can anyone advise on my phagocytosis of influenza virus by Raw 264.7 culture using Vybrant phagocytosis assay kit?

I have Raw 264.7 culture which are healthy. I infect them with influenza virus and then stimulate the cells using LPS. I incubate them for 24hrs and carryout phagocytosis assay using Vybrant...

23 July 2014 2,747 2 View