Keegan Alexander Smith

4 Questions 10 Answers 0 Followers

Questions related from Keegan Alexander Smith

How can I create a "search within radius" tool in ArcGIS 10?

I'm creating a set of tools for the ENGO I'm working with, using ESRI ArcGIS 10. The deliverable that I'm having some trouble with is this: We want a tool that will search for a specific feature...

04 April 2014 9,953 3 View

Organics-based DNA Extractions without access to a fumehood?

Hello everyone, in our lab we have been performing CTAB/phenol-chloroform DNA extractions. They're time-consuming (especially for our level of throughput) but they give us lots of clean DNA, for...

09 September 2013 1,960 7 View

PCR cycling conditions: combining annealing and extension steps?

I'm trying to optimize my cycling conditions for my PCR assay, and I've encountered some divergence in the literature. Some investigators seem to think that annealing (which I'm carrying out at...

08 August 2013 5,795 48 View

How can I account for biological replication in my standard curve for calculating qPCR efficiency?

We have isolated genomic DNA from three biological replicates (3 different petri plates of filamentous fungi) of our samples (2 treatments and a control). We are then proceeding to qPCR, examining...

08 August 2013 5,421 5 View