Keegan Alexander Smith

4 Questions 5 Answers 0 Followers

Questions related from Keegan Alexander Smith

50% nitric acid bath container?

We're using a fluorometer (Horiba Aqualog) to analyze fluorescent dissolved organic matter (FDOM) in water samples. The samples are analyzed in quartz cuvettes, which must be bathed in a solution...

18 November 2020 7,405 2 View

Organics-based DNA Extractions without access to a fumehood?

Hello everyone, in our lab we have been performing CTAB/phenol-chloroform DNA extractions. They're time-consuming (especially for our level of throughput) but they give us lots of clean DNA, for...

12 September 2013 7,734 6 View

PCR cycling conditions: combining annealing and extension steps?

I'm trying to optimize my cycling conditions for my PCR assay, and I've encountered some divergence in the literature. Some investigators seem to think that annealing (which I'm carrying out at...

14 August 2013 3,301 46 View

How can I account for biological replication in my standard curve for calculating qPCR efficiency?

We have isolated genomic DNA from three biological replicates (3 different petri plates of filamentous fungi) of our samples (2 treatments and a control). We are then proceeding to qPCR, examining...

01 August 2013 8,483 4 View