Kayla Simeone

3 Questions 3 Answers 0 Followers

Questions related from Kayla Simeone

Protein extraction from micro-dissected tissues?

I have been using a classic lysis buffer in order to extract proteins from a micro-dissected tumor tissue (300µm by 400µm). I have tried dissociating the tissue with 1) collagenase 2) lysis buffer...

04 April 2016 548 2 View

RNA extraction of Micro dissected tissues?

Hi, I've tried to extract RNA from my micro-dissected tissues and I've been getting small RNA concentrations and very poor A260/A280 ratio between 0.6-1.47. I've tried RNA extraction using 1....

03 March 2016 7,974 7 View

For Micro-dissected tissue fixation, is it better to use Formalin or Paraformaldehyde and for how long should I incubate for ?

I am presently using 10% formalin for fixation of the micro-dissected tissues. They are 300µm in height and a diameter of approximately 420µm. We are presently trying various test at different...

03 March 2016 1,045 6 View