Katy A Lloyd

2 Questions 3 Answers 0 Followers

Questions related from Katy A Lloyd

What is the best buffer to use for Fluo-4 AM staining of lymphocytes?

I want to investigate calcium mobilization of lymphocytes (B cells and T cells) upon receptor ligation. I have purchased some Fluo-4 AM to do this, and when researching possible staining protocols...

06 June 2014 8,593 3 View

What is the best method for fixing non adherent cells to microscope slides?

I want to know which are the best methods for fixing non adherent cells to microscope slides/coverslips for immunofluorescence microscopy? I have tried using a cytospin, but was wondering if there...

09 September 2013 3,782 3 View