Katherine Duchesneau

4 Questions 1 Answers 0 Followers

Questions related from Katherine Duchesneau

How can I extract mycorrhizal DNA from plant roots for whole genome sequencing while decreasing the number of plant reads?

We are using illumina and have access to both the qiagen dneasy kit and the powersoil kit from mobio (I am also extracting from soil). Thank you!

18 January 2017 3,621 2 View

Does anyone know of a good protocol to inoculate seedlings with multiple species of arbuscular mycorrhizal fungi from spore?

I am doing a glasshouse experiment and need to inoculate seedlings with spores of multiple AMF species extracted from soil. I would like more than one species to colonize the seedling...

29 October 2016 767 3 View

What are the advantages of using next generation sequencing (illumina) for a metagenomics study over the use of TRFLP?

To conduct a soil experiment on microbial composition as well as mycorrhizal fungal composition, what are the advantages/ disadvantages of using illumina over TRFLP?

20 September 2016 8,428 8 View

What is the best method to preserve soil samples and their microbial communities for later analysis (eg. hyphal length and AMF spore extraction)?

I would like to preserve my soil samples (about 30g of soil per sample) and analyze them later (in a few months). Would anyone have any particular recommendation? I have already air dried them...

06 September 2016 8,040 7 View