Kat Hopkinson

2 Questions 1 Answers 0 Followers

Questions related from Kat Hopkinson

Can I spike DNA samples with gene fragment as internal amplification control for real time qpcr?

I have DNA samples that have been extracted from stool samples, which I am running qpcr on for diagnostics. I was wondering what to use as an internal amplification control, since stool has a lot...

21 June 2022 7,722 2 View

Whats the best way to extract DNA from dried blood spots on FTA cards? How much DNA is expected to be on cards?

I'm trying to optimize process of DNA extraction from dried blood spots on FTA cards for qPCR or PCR, for diagnostic purposes. I've tried Qiagen DNA mini kit, and Alkaline extraction. I've also...

10 December 2021 350 0 View