10 Questions 31 Answers 0 Followers
Questions related from Kashif Shamim
While checking the effect of inhibitors on protease enzyme it was found that iodoacetate instead of inhibiting the protease activity it has enhanced the protease activity. I searched alot but...
03 March 2018 9,477 5 View
I would like to amplify 4 kb fragment of gene of interest. In order to do so I am little bit confused as to whether I should go for normal Taq DNA pol or some thing else. My point is, will normal...
01 January 2016 8,685 5 View
My product size is of 194 bb but i am getting non specific band of more than 300 bp. Moreover, as compared to my gene of interest of 194 bp which gives me faint band. I tried with gradient pcr but...
02 February 2014 1,044 21 View
I have a pET expression series of vectors but when I went through the construct I found that non of them had a blunt end restriction digestion site at MCS of the vector. How to clone a PCR product...
06 June 2013 5,293 23 View
Like other courses in India, can the course duration for a Ph.D be fixed for a particular time period?
12 December 2012 6,733 21 View
What are the merits and demerits of primer binding inside and outside the gene of interest?
12 December 2012 6,526 11 View
I would like to clone a gene which codes for restriction enzyme Sau 3AI but I am not getting the primer sequence. Can anyone help?
12 December 2012 3,573 5 View
I hardly get 3 to 4 colonies per plate.
11 November 2012 7,681 7 View
I get 5 bands of my protease enzyme out of which I have to purify the band which is responsible for protease activity and then I want to go for its identification.
10 October 2012 6,226 5 View
I wanted to purify and isolate my DNA from agarose gel, so I wish to know what type of gel and what percentage of agarose I can add to make the gel and how to isolate DNA after the run. Apart from...
10 October 2012 480 11 View
