Karol Urbanek

3 Questions 1 Answers 0 Followers

Questions related from Karol Urbanek

What is the best anti-APC antibody for FACS?

Dear all, I'm looking for anti-human APC (Adenomatous Polyposis Coli) antibody for FACS. Preferably stained. Since APC also stands for fluorophore, I have a really hard time finding any. I used...

05 September 2022 1,424 1 View

Did anyone try Accell siRNA in MCL cell lines?

Hello Everyone, Currently, I am trying to silence a gene in a panel of CLL and MCL cell lines. In my first attempt KD efficiency ranged from 50 to 75% (assessed by qPCR, I have not looked at...

26 May 2022 8,879 2 View

Does the ratio of non-phospho B-catenin to total B-catenin can be used as a marker of WNT activation?

I want to compare WNT activity status in a few lymphoma cell lines and I'm wondering if I can use B-catenin levels (via western blot or FACS) to do it? I know that the right way to go Is to do TOP...

07 January 2022 6,393 1 View