Kanti K Yadav

4 Questions 10 Answers 0 Followers

Questions related from Kanti K Yadav

How can I troubleshoot protein precipitation after purification?

Hello everyone, I have been trying to express and purify a protein. I am getting a good protein yield after Ni-NTA chromatography, as seen in SDS-PAGE. Problem is after purification, during...

05 August 2015 9,706 7 View

Can dialysis membranes be reused?

Hello, I have to perform dialysis after Ni-NTA purification. Can I reuse a dialysis membrane. It has been kept in a sodium azide solution(1%) fir past six months at 4 degree. If yes, what is the...

10 July 2015 3,862 9 View

Can anybody help me with purification of a his tagged protein?

hi, I am trying to express and purify a His-tagged protein of interest. I am using pET vector and BL21DE3 strain for expression. At first, I was able to express the protein with .5mM IPTG for 5h...

21 April 2015 6,298 3 View

How can I know if my protein a monomer or dimer?

I am working on a protein. In litertaure it has been described as a monomer, even on SDS-PAGE a single band is observed. For docking studies, when I took the PDB file of the protein, it shows it...

21 April 2015 6,676 9 View