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Questions related from Kanti K Yadav
Hello everyone, I have been trying to express and purify a protein. I am getting a good protein yield after Ni-NTA chromatography, as seen in SDS-PAGE. Problem is after purification, during...
05 August 2015 9,681 7 View
Hello, I have to perform dialysis after Ni-NTA purification. Can I reuse a dialysis membrane. It has been kept in a sodium azide solution(1%) fir past six months at 4 degree. If yes, what is the...
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hi, I am trying to express and purify a His-tagged protein of interest. I am using pET vector and BL21DE3 strain for expression. At first, I was able to express the protein with .5mM IPTG for 5h...
21 April 2015 6,254 3 View
