Kanthalu Shagadevan Dinesh Babu

3 Questions 10 Answers 0 Followers

Questions related from Kanthalu Shagadevan Dinesh Babu

How to get cDNA from long mRNA molecules ?

My gene of interest is 5.5 kb long one. this gene has been expressed only under stress conditions and I have used NaCl treatment (Salinity stress) to induce the gene expression and I have isolated...

03 March 2016 7,693 5 View

Why is my Genei Taq polymerase not amplifying a particular cDNA using RACE protocol?

10x Taq buffer and MgCl2 were provided separately, and I am using these at 1x concentration. Positive control and water controls are working good, but the target cDNA has not yet been amplified.

10 October 2014 5,594 11 View

Dear sir I want to amplify a cDNA using pfu polymerase. pfu amplifies the target cDNA but it doesnot amplifies the positive control plasmid DNA ?

I am using Fermentas pfu polymerase. it is provided with single buffer vial which has MgSo4 also.

10 October 2014 6,751 3 View