Kamila Weissova

4 Questions 3 Answers 0 Followers

Questions related from Kamila Weissova

How can cell immortalization affect gene expression?

how can be changed gene expression in imortalized lymphoblasoid cells? The lymphoblasoid were get from PBCM and imortalized by EB virus. THX

18 July 2016 8,677 1 View

Can I use as a housekeeping gene for miRNA normalizatinon mRNA houskeeping gene (for example GAPDH)?

I didn´t find anything that can be used as a housekeeping gene for miRNA (miR-219) for PCR..but can I just use commonly used GAPDH?

16 January 2015 2,145 3 View

What can I use as a positive control and standards for miRNA from blood samples in PCR?

I would like to detect specific miRNA(miR-219) in human blood, I have no idea what can I use as a positive control.

14 January 2015 1,050 4 View

Can I use same PCR protocol using Taqman assay for mRNA and miRNA detection?

So Can I use 5X HOT FIREPOL PROBE ROX for miRNA detection? I´m get used to use that FIREPOL for mRNA detection, but i never tried that for miRNA...THX

14 January 2015 7,478 3 View