Kamal Adhikari

5 Questions 5 Answers 0 Followers

Questions related from Kamal Adhikari

Is there any Cytotoxicity assay that has been reported for the novel polyketides product generated by engineering plant Polyketide Synthase Type III?

I am engineering polyketide synthase III to generate unnatural (novel) Polyketides products. I would like to test its cytotoxicity. Is there any PKS-III novel product use for cytotoxicity assay...

12 December 2015 9,930 2 View

Can we use MTT-assay for high-throughput screening (HTS) for determining the cytotoxicity of drugs?

I am curious whether MTT can be use for HTS or not? i need to determine the cytotoxicity of 50 different small compounds against various panel of cancer cell line (7 cancer cell line). Is it...

07 July 2015 3,418 4 View

Is there any software to calculate the active site cavity of a protein?

My protein doesn't have complex structure . So, right now i am doing homo-logy modelling with other protein (with 74% sequence identity). Does, this methods give us the idea to know the active...

06 June 2015 8,164 3 View

Problem on Expression of arabidopsis thaliana 4-coumaroyl--coa ligase 3 (At4CL3) in E.coli??

I am using Arabidopsis thaliana gene (4-coumaroyl-coa ligase 3) At4CL3 to express through E,coli strain BL21 (De3) strain. I used Pet28a expression vector to express the protein with 6xhis tag. I...

11 November 2014 5,162 4 View

Is using Thermos Scientific Phusion Flash High-Fidelity PCR master Mix as my DNA polymerase applicable for DNMT1 site-directed Mutagenesis?

I am doing DNMT1 site-directed Mutagenesis (using PCR) using Thermos Scientific Phusion Flash High-Fidelity PCR master Mix as my DNA polymerase to mutate my desired side. After PCR and gel...

09 September 2012 3,533 1 View