Kamal Malhotra

5 Questions 20 Answers 0 Followers

Questions related from Kamal Malhotra

Which counter ion (among acetate or formate) is recommended to replace TFA after peptide synethsis?

Hi, Can anyne suggest which is best counter ion (acetate/formate) to replace TFA after peptide synthesis when one has to work on cells and toxicity of TFA is a concern?

14 August 2019 3,367 7 View

I am trying to dissolve Fmoc FF at higher pH but it is not completely soluble. Please suggest

I am facing difficulty in solubility of Fmoc FF in water. I am taking 10mM peptide and trying to dissolve in water at pH 10. But it is not completely soluble but several papers report its...

07 July 2017 1,837 4 View

Can anyone suggest what could be the possible cause of a wavy baseline in HPLC?

I am doing peptide RP-HPLC but it gives noise and wavy baseline. What could be the probable cause of it and can anybody suggest a solution for that?

04 March 2015 3,661 23 View

Why would all prefer to use female athymic mice for invivo studies related to non-small cell lung cancer?

I have seen in most of the research articles that female nude (athymic) mice has been used mostly for invivo studies related to non small cell lung cancer. I have a question that why they used...

22 September 2014 4,996 5 View

Does targeting to lysosome require targeting moiety and what would be the fate of any peptide taken up by a cell?

Is it true that any molecule like peptide is taken up by cell is through lysosome only. So if I need to target any molecule to lysosome and then want it to release into cytosol do it require the...

14 August 2014 4,309 8 View