Kalyan Mahapatra

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Questions related from Kalyan Mahapatra

How to prepare H2DCFDA solution for ROS imagong?

I am looking forward to perform ROS imaging in Arabidopsis thaliana roots using H2DCFDA. I am not sure which solvent should I use for dissolving DCFDA and what should be the ideal concentration...

22 September 2019 188 4 View

How to determine the N-terminal and C-teeminal components for BiFC assay?

I am planning BiFC assay to check the interaction of a transcription factor with another protein. I shall use pSATN vectors. Should the transcription factor be used for N terminal or C terminal...

17 June 2019 4,179 0 View

How to clone a promoter region of a gene within pBI101.2 vector for GUS assay?

I am cloning about 1.5kb promoter of a gene within pBI101.2 vector for GUS assay. During the primer designing, I am facing a problem. Should I include some portion(say about +20 to +30 from the...

11 June 2019 7,115 2 View